Monika Gaik

My scientific education took root during my undergraduate studies at the Jagiellonian University, an experience enriched by a transformative internship at Cambridge University (UK), where I gained intensive training in protein biochemistry. This further ignited my passion for the life sciences and set me on the path of advanced research. I subsequently pursued my PhD at Ruprecht Karls University in Heidelberg, Germany, where I focused on deciphering the molecular architecture of the nuclear pore complex (NPC) – a large macromolecular machine responsible for the selective transport of molecules between the nucleus and cytoplasm. This project resulted with my lead-author publication in a high-impact journal (Gaik et al., JCB).

I joined the Max Planck Research Group in Poland, where I set my research focus at molecular medicine and structural biology. I secured funding through POLONEZ NCN grant for researchers returning to Poland and SONATA BIS 14 NCN grant (2025-2029). My first independent project focused on understanding how mRNA molecules and their associated RNA-binding proteins coordinate protein synthesis during stem cell differentiation – a process whose dysregulation has been linked to diseases such as cancer and infertility. Driven by curiosity about the role of RNA modifications in cellular function, I subsequently investigated the Elongator (ELP) complex – responsible for modifying uridine at position 34 of several tRNAs – and its connection to neurodevelopment. Focusing on clinically relevant, patient-derived mutations in the ELP456 subcomplex, I applied cryo-EM technique and biochemical assays to assess their structural and functional impact. Collaborative efforts enabled me to establish the first clinical link between defects in the ELP456 subcomplex with neurodevelopmental disorders, characterized by an inability to reach cognitive and motor milestones. By determining the cryo-EM structures of human and murine Elp456 subcomplexes, I pinpointed critical mutated residues, thereby providing novel insights into how altered tRNA modifications can impair protein production and neural function (Gaik et al., EMBO Mol Med). By applying the crystallographic fragment screening, I am currently developing novel research tools that can eventually be transformed into pro-drugs for RNA modopathies, thereby paving the way for new therapeutic strategies against previously incurable diseases. I embrace an interdisciplinary perspective that is committed not only to understanding fundamental biological processes but also to converting these discoveries into clinical applications.

ORCID: https://orcid.org/0000-0003-4939-3821

Grants

  1. Sonata Bis 14 grant (2025-2029) funded by the National Science Centre (NCN), Poland Title: “Molecular analyses of human dihydrouridine synthases acting on tRNA”. Malopolska Centre of Biotechnology of the Jagiellonian University, Cracow, Poland
  2. Polonez 1 grant (2016-2018) funded by the National Science Centre (NCN), Poland and European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 665778. Grant no: 2015/19/P/NZ1/02514 & No. 665778 Title: “Structural insights into translational control of nanos mRNA by the Sex lethal repressive complex”. Malopolska Centre of Biotechnology of the Jagiellonian University, Cracow, Poland

Awards and Fellowships

  • 2023/11 Rector’s Prize of the Jagiellonian University for outstanding researchers, Cracow, Poland
  • 2018/09 Travel grant for attending the EMBO Practical Course on “Cryo-Electron Microscopy and 3D Image Processing”, Heidelberg, Germany.
  • 2018/06 Best workshop Award at Children’s University Foundation work the best workshop entitled:“How to invent a new drug?”, Cracow, Poland.
  • 2013/10 Recipient of the Fellowship from German Ministry of Education and Research Fellowship within “Experimental biomedical research cluster” category in Baden-Wurttemberg region, Germany.
  • 2010/07 Award for completing first Thesis Advisory Meeting of PhD Thesis from the  Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology (HBIGS), Heidelberg, Germany       
  • 2008 Internship at University of Cambridge, United Kingdom, Laboratory of Molecular Biology (LMB), under supervision of Professor Alan Warren

Scientific activities

  • 2025 Speaker at the 2nd Polish RNA Biology Meeting in Poznan, Poland
  • 2024 Speaker at the 26th Heart of Europe Crystallography (HEC) Meeting in Cracow, Poland
  • 2021 Speaker at the Golden seminar at MCB of the Jagiellonian University, Poland
  • 2018 Invited speaker at the University of Regensburg. Lecture title: “Uncovering molecular mechanisms of translational control with structural biology”, Regensburg, Germany
  • 2018 EMBO Practical Course: “Cryo-Electron Microscopy and 3D Image Processing”, Heidelberg, Germany
  • 2018 Participating and co-organizing the Małopolska Researchers’ Night, Kraków, Poland
  • 2017 Conference on methods and applications in the frontier between MX and CryoEM, Barcelona, Spain
  • 2017 International Crystallography School (AICS2017). “Bridging the gap between cryo-EM and crystallography”, Pavia, Italy
  • 2017 The 22nd Annual Meeting of the RNA Society, Prague, Czech Republic. Poster presentation: “RNA-binding proteins influence stem cells’ fate decisions”
  • 2017 Polish-German Science Meeting, Munich, Germany
  • Representing Max Planck Research Group to discuss current program and new strategies for implementing excellence in Germany and Poland
  • 2017-2018 – Supervising the Master student from Jagiellonian University in Krakow, Poland, project title: “The impact of disease-related mutations on the Elp2 protein stability”.
  • 2017 EMBO Polish Workshop on Computational and Structural Biology, Serock, Poland. Selected speaker: “Uncovering molecular mechanisms of translational control with structural biology”
  • 2016 Marie Curie Fellow Member of Marie Curie Alumni Association and active participant in its Polish Chapter. The main goal is to attract more members and initiate fruitful exchange of idea between scientists from diverse research fields.
  • 2016 – present – supervising a PhD student within Polonez 1 Marie Curie Fellowship, project title: “Structural insights into translational control of nanos mRNA by the Sex lethal repressive complex
  • 2016 – Supervising the Master student from Vienna University, Austria, project title: “Cloning and expression of CCR4-NOT component Twin protein from Drosophila”.
  • 2016 Interview for Lab Times Magazine Promoting developing life sciences in Poland in the article: ‘Let’s not Waste this Chance!‘ Gaik M, Glatt S, Grudnik P, Heddle J, Tarnwaska K, Yamada K.
  • 2012 International Spring Meeting, Bad Hindelang, Germany. Selected speaker, presentation title: “A translation-controllable pulse-chase method to study ribosome biogenesis and nuclear pore complex assembly in vivo“,
  • 2012 Annual Meeting of Graduate School – HBIGS, Heidelberg, Germany Poster presentation at the Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology (HBIGS). Title: “Insight into in vivo assembly of Nuclear Pore Complex (NPC) by means of translation- controllable pulse-chase method
  • 2012 and 2013 – Teaching practical courses for students of Molecular and Cellular Biology at Heidelberg University entitled: “Biochemical research on proteins, lipids and nucleic acids – in vivo purification and in vitro reconstitution of protein complexes”.
  • 2011 Symposium on Dynamics of Macromolecular Complexes in Biosynthetic Transport, Heidelberg, Germany. Poster presentation at SFB638 grant proposal, entitled “Pulse-chase analysis of TREX-2 and SAGA factors to determine their interconnection during transcription- coupled mRNA export
  • 2011 – Supervising the Master student from Molecular and Cellular Biology program at Heidelberg University, project title: “Investigating the function of SAGA and TREX-2 in transcription-coupled mRNA export, with a particular focus on the Sus1 protein”.

Publications

  • Hammermeister A, Gaik M, Dahate P, Glatt S. Structural Snapshots of human tRNA Modifying Enzymes, Journal of Molecular Biology (JMB) 2025 Aug 15, 437, 16, https://doi.org/10.1016/j.jmb.2025.169106
  • Jaciuk M, Scherf D, Kaszuba K, Gaik M, Kocielniak A, Krutyhołowa R, Rawski M, Indyka P, Biela A, Dobosz D, Lin TY, Abbassi NEH, Hammermeister A, Rappsilber J, Kosinski J, Schaffrath R and Glatt S. Cryo-EM structure of the fully assembled eukaryotic Elongator complex. Nucleic Acids Res. (NAR) 2023 Jan 9:21;51(5):2011-2032 DOI 1093/nar/gkac1232
  • Jain S, Koziej L, Poulis P, Kaczmarczyk I, Gaik M, Rawski M, Ranjan N, Glatt S, Rodnina M. „Modulation of translational decoding by m6A modification of mRNA”. Nature Communications, 2023 Aug 8; 14(1):4784. PMID: 37553384; PMCID: PMC10409866. DOI 1038/s41467-023-40422-7
  • Gaik M, Kojic M, Wainwright BJ, Glatt S. Elongator and the role of its subcomplexes in human diseases. EMBO Molecular Medicine. 2023 Feb 8;15(2):e16418. DOI: 15252/emmm.202216418
  • Gaik M, Kojic M, Stegeman MR, Öncü-Öner T, Kościelniak A, Jones A, Mohamed A, Chau PYS, Sharmin S, Chramiec-Głąbik A, Indyka P, Rawski M, Biela A, Dobosz D, Millar A, Chau V, Ünalp A, Piper M, Bellingham MC, Eichler EE, Nickerson DA, Güleryüz H, Abbassi NEH, Jazgar K, Davis MJ, Mercimek-Andrews S, Cingöz S, Wainwright BJ, Glatt S. Functional divergence of the two Elongator subcomplexes during neurodevelopment. EMBO Molecular Medicine. 2022 Jul 7;14(7):e15608. DOI: 15252/emmm.202115608
  • Salerno-Kochan A, Horn A, Ghosh P, Nithin C, Kościelniak A, Meindl A, Strauss D, Krutyhołowa R, Rossbach O, Bujnicki JM, Gaik M#, Medenbach J#, Glatt S#. Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26. Life Sci Alliance (LSA). 2022 May 5;5(8):e202201418. PMID: 35512835 (# corresponding author). DOI 26508/lsa.202201418
  • Kojic, M, Gawda, T, Gaik, M, Begg A, Salerno-Kochan A, Kurniawan ND, Jones A, Drożdżyk K, Kościelniak A, Chramiec-Głąbik A, Hediyeh-Zadeh S, Kasherman M, Shim WJ, Sinniah E, Genovesi LA, Abrahamsen RK, Fenger CD, Madsen CG, Cohen JS, Fatemi A, Stark Z, Lunke S, Lee J, Hansen JK, Boxill MF, Keren B, Marey I, Saenz MS, Brown K, Alexander SA, Mureev S, Batzilla A, Davis MJ, Piper M, Bodén M, Burne THJ, Palpant NJ, Møller RS, Glatt S, Wainwright BJ. Elp2 mutations perturb the epitranscriptome and lead to a complex neurodevelopmental phenotype. Nat Commun. 2021 May 11;12(1):2678. DOI 1038/s41467-021-22888-5
  • Boutard N, Bialas A, Sabiniarz A, Guzik P, Banaszak K, Biela A, Bien M, Buda A, Bugaj B, Cieluch E, Cierpich A, Dudek L, Eggenweiler HM, Fogt J, Gaik M, Gondela A, et al. Discovery and Structure-Activity Relationships of N-Aryl 6-Aminoquinoxalines as Potent PFKFB3 Kinase Inhibitors. ChemMedChem. 2019 Jan 8;14(1):169-181. DOI: 1002/cmdc.201800569
  • Boutard N, Bialas A, Sabiniarz A, Guzik P, Banaszak K, Biela A, Bien M, Buda A, Bugaj B, Cieluch E, Cierpich A, Dudek L, Eggenweiler HM, Fogt J, Gaik M, et al. Synthesis of amide and sulfonamide substituted N-aryl 6-aminoquinoxalines as PFKFB3 inhibitors with improved physicochemical properties. Bioorganic Medicine Chemistry Letters. 2019 Feb 15;29(4):646-653. doi: 1016/j.bmcl.2018.12.034
  • Kojic M, Gaik M, Kiska B, Salerno-Kochan A, Hunt S, Tedoldi A, Mureev S, Jones A, Whittle B, Genovesi LA, Adolphe C, Brown D, Stow J, Alexandrov K, Sah P, Glatt S, Wainwright B J. Elongator mutation in mice induces neurodegeneration and ataxia-like behaviour. (Nat Commun.), 2018 Aug 10;9(1): 3195; IF: 12.353
  • Moschall R, Gaik M, Medenbach J, Promiscuity in post-transcriptional control of gene expression: Drosophila Sex Lethal and its regulatory partnership. FEBS Letters (FEBS Lett), 2017 Jun; 591(11): p.1471-1488; IF: 2.999
  • Gaik M, Flemming D, von Appen A, Kastritis P, Mucke N, Fischer J, Stelter P, Ori A, Bui K H, Bassler J, Barbar E, Beck M, Hurt E. Structural basis for assembly and function of the Nup82 complex in the nuclear pore scaffold. The Journal of Cell Biology (JCB), 2015 Feb 2; 208 (3): p.283- 297; IF: 8.717
  • Horwacik, I*, Gaik, M*, Durbas, M, Boratyn, E, Zając, G, Szychowska, K, Szczodrak, M, Kołoczek, H, Rokita, H. Inhibition of autophagy by 3-methyladenine potentiates sulforaphane-induced cell death of human neuroblastomaBE(2)-C cell line.Molecular Medicine Reports (Mol. Med. Rep.), 2015 Jul; 12(1): p.535-542; IF: 1.559
  • Stelter P, Kunze R, Radwan M, Thomson E, Thierbach K, Thoms M, Hurt E. Monitoring Spatiotemporal Biogenesis of Macromolecular Assemblies by Pulse-Chase Epitope Labeling. Molecular Cell (Mol Cell), 2012 47,(5), p. 788-796. (under maiden name Monika Radwan); IF: 15.28